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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could lead to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital job in advertising and marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two added splice variants were claimed but specific nucleotide sequence information and facts isn't however out there.
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Blocking peptides are peptides that bind specifically into the goal antibody and block antibody binding. These peptide commonly incorporates the epitope identified with the antibody.
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This guide outlines further more attainable optimization of cellular and immunoassay parameters to be certain the absolute best outcomes are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining with the blocked antibody vs . the antibody by itself, one can see which staining is certain.
Goal Facts Cyclin E belongs to the extremely conserved cyclin loved ones, whose associates are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is particularly degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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